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. 2022 Mar 30;23(7):3832. doi: 10.3390/ijms23073832

Figure 1.

Figure 1

Genome-wide identification and expression profiles of the HEX genes in B. mori. (A) Gene structure and (B) protein domains of nine GH20 HEX genes identified in B. mori. Glycohydro 20b2 and Glyco hydro 20 are abbreviations of glycoside hydrolase 20b2 and glycoside hydrolase 20 in SMART. (C) Phylogenetic analysis of HEXs of B. mori and other species. The phylogenetic tree was constructed using neighbor-joining method and bootstrap support values on 1000 replicates by MEGA7. The HEX proteins in B. mori are labeled with a red line. (D) Heatmap of the expression level of eight HEX genes in 12 tissues (from the outside to the inside: anterior silk gland, epidermis, fat body, head, hemolymph, Mal-pighian tubule, middle silk gland, midgut, ovary, posterior silk gland, testis, and trachea) on day three of the fifth instar and wandering stage. Red dots indicate epidermis. 5L3D: day three of fifth instar and W: wandering stage. (E) Heatmap of the expression level of eight HEX genes in seven developmental stages (from the outside to the inside: day three of fourth instar; molting phase in the fourth instar; the start of the fifth instar; day three of fifth instar; W, wandering stage; PP, pre-pupa stage; and P1, day one of pupa) of the epidermis, ovary, and testis in Bombyx mori. Blue indicates low expression and red indicates high expression.