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. 2022 Mar 22;23(7):3417. doi: 10.3390/ijms23073417

Figure 7.

Figure 7

Immunohistochemistry and fibrinogen-positive area in lungs. (A) Lung tissue sections in the control, nontreated, and acute lung injury (ALI) groups after 2D-LFB-Sec, 3D-LFB-Sec, 2D-MSC-Sec, and 3D-MSC-Sec inhalation. Sections were stained with antibodies against fibrinogen, and cell nuclei were labeled with hematoxylin; ×20 magnification; scale bars = 100 μm. (B) The percentage of fibrinogen-positive areas in the total area of lung tissue in the control, nontreated, and ALI groups after 2D-LFB-Sec, 3D-LFB-Sec, 2D-MSC-Sec, and 3D-MSC-Sec inhalation. Data are presented as the mean ± SEM (n = 54). ****, adjusted p < 0.0001 for Intact vs. Control ALI, 2D-LFB-Sec vs. Control ALI, 3D-LFB-Sec vs. Control ALI, 2D-MSC-Sec vs. Control ALI, and 3D-MSC-Sec vs. Control ALI. (ns) represents a nonsignificant difference for multiple comparisons between 2D-LFB-Sec, 3D-LFB-Sec, 2D-MSC-Sec, and 3D-MSC-sec. Analyses were performed using Brown–Forsythe and Welch analysis of variance (ANOVA) with the Games–Howell post hoc test. ALI, acute lung injury; MSC, mesenchymal stromal cell; LFB, lung fibroblast.