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. 2022 Mar 22;23(7):3461. doi: 10.3390/ijms23073461

Figure 3.

Figure 3

Characterization of Long Interspersed Element-1 (LINE-1)-containing extracellular vesicles (EVs). We examined EVs from the density gradient fractions shown in Figure 2 using Nanosight Nanoparticle tracking analysis (NTA). (A) NTA profiles of all fractions. (B) ORF1p-rich fractions. ORF1p was consistently found in EVs with a density greater than ~1.12 g/mL, most often in Fractions 10 and 11 (~1.16–1.2 g/mL). (C) LINE-1 mRNA-rich fractions. LINE-1 mRNA was detected in EVs with low-to-moderate density (~1.03–1.07). (D) β-Actin-rich fractions. Moderate-density fractions with the highest enrichment for β-Actin are shown for comparison. Mean of five readings ± SE shown.