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Methylation of ENPP2 estimated by qMSP in ccfDNA (A) from BrCa patients and healthy individuals (B) from each group separately (adjuvant, metastatic, neoadjuvant, and healthy). Abbreviations: ccfDNA: circulating cell-free DNA; qMSP: quantitative methylation-specific PCR; CRC: colorectal cancer. * Control in relation to Adjuvant, # Control in relation to Metastatic, $ Control in relation to Neoadjuvant.
