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Primer sequences, annealing temperatures, and genomic locations used for qMSP assays.
| GENE | Primer Sequence (5′–3′) | Annealing Temperature (°C) | Product Length | Genomic Loci |
|---|---|---|---|---|
| ENPP2 | MET F: CGTTTTTTTATTTGATACGATTGGAACGA MET R: CAAAACCTCAAAACAATACACTCCGTAA |
60 | 117bp | Chr8: 120650976- 120651092 (+1 strand) |
| ACTB | F: TGGTGATGGAGGAGGTTTAGTAAG R: AACCAATAAAACCTACTCCTCCC |
60 | 134bp | chr7: 5558705– 5558838 (−1 strand) |
Abbreviations: MET: methylated, F: forward, R: reverse.
