FIG. 3.

Cellular leakage of 260-nm-absorbing materials. The following compounds were added to cells washed with MOPS buffer (50 mM, pH 6.0) and incubated for the specified times before the extracellular fractions were collected: DMSO (control) (●, ○), polygodial at 25 μg/ml (▴, ▵), and polygodial at 6.25 μg/ml (▾, ▿) (A), and amphotericin B at 6.25 μg/ml (■, □), amphotericin B at 1.56 μg/ml (⧫, ◊), and miconazole at 25 μg/ml ( , ) (B). Filled symbols represent the compound, and open symbols represent the compound plus 10 mM Ca²⁺.