TABLE 1.

MICs and MFCs of polygodial with calcium and EDTA against S. cerevisiae

Treatment	ME		RPMI 1640
	MICa	MFCb	MIC	MFC
Polygodial	1.56	3.13	3.13	6.25
+1 mM EDTA	—c	0.78	—	—
+0.2 mM EDTA	0.4	1.56	—	—
+100 mM CaCl2	0.78	1.56	0.02	0.02
+10 mM CaCl2	1.56	3.13	0.02	6.25
+1 mM CaCl2	1.56	3.13	3.13	6.25

^aMicrograms per milliliter. Determined by the NCCLS microbroth dilution method M27-A. 

^bMicrograms per milliliter. Determined by transferring 5 μl from the MIC wells to fresh media. 

^c—, cells did not show growth in the absence of polygodial.