Figure 4.

Topical application of ERK inhibitor improved TEWL and restored FLG expression in mite antigen-induced AD in mice. (A) TEWL was measured on the lesion by a Vapo Scan instrument on day 0, 2, 4, and 6. Data were presented as the average of five points repeated recordings. All data are presented as mean ± S.E.M. (n = 9 per group). *** p < 0.001 Student’s unpaired two-tailed t-test on each day. Immunohistochemical analysis of FLG protein (green) in the back skin in control (B), AD (C), AD + U0126 (D) groups on day 7. Nuclei were counterstained with DAPI (blue). Scale bar: 50 μm.