Figure 5.

ERK inhibitor restored the reduction in the expression levels of filaggrin and involucrin in NHEK. NHEKs were treated with IL-4 (20 ng/mL) in the presence or absence of ERK inhibitor (1 µM) for 72 h (FLG) or 48 h (IVL) for qRT-PCR and for 96 h (FLG) or 72 h (IVL) for Western blotting analyses. FLG (A) and IVL (B) mRNA expression was analyzed by qRT-PCR. FLG (A) and IVL (B) protein expression was analyzed by Western blotting. All data are presented as mean ± S.E.M. (n = 3 per each group). ** p < 0.005, *** p < 0.001 one-way analysis of variance followed by Bonferroni’s multiple comparison test.