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ERK inhibitor inhibited chemokine production from BMDC induced by IL-4. BMDCs were treated with IL-4 (20 ng/mL) in the presence or absence of ERK inhibitor. (A,B) CCL17 and CCL22 mRNA expression was analyzed by qRT-PCR. (C,D) CCL17 and CCL22 protein expression was analyzed by ELISA. All data are presented as mean ± S.E.M. (n = 3 per each group). * p < 0.05, ** p < 0.005, *** p < 0.001 one-way analysis of variance followed by Bonferroni’s multiple comparison test.
