Figure 7.

Quantification of the results. (A). Fluorescence intensity of VSVG dots does not change during intra-Golgi transport. (B) Proportion of PCI and VLDL distensions separated with the pore row is high. (C,D) Proportions of cisternal distensions surrounded with pores. Pores surround cisternal distensions with PCI (C) and VLDL (D) in both the first and last Golgi cisternae. (E–H) Distribution of gold labelling for PC distensions (E,F) and VSVG (G,H) at 2 min after release of transport block, according to the mini-wave (E,G) and maxi-wave (F,H) protocols. Labelling densities of gold and distensions in the first medial cisterna in (E,G) are significantly higher than in the last medial cisterna (p < 0.05), whereas this difference is less in (F,H). (I) Fluorescence recovery (dynamics of the ratio between fluorescence intensity between the bleached zone and ER). Synchronisation protocols and times after the release of the temperature blocks are indicated in the images. After the maxi-wave synchronisation, the recovery is greater at 6 min than those during the mini-wave and at 12 min after release using the maxi-wave. (I,J) Inhibition of the COPI function decreases the rate of pore restoration decrease. In CHO and ldlF cells, the COPI function was inhibited by heating cells to 40 °C for 2 min. In CHO cells, the bar after is lower than the bar resting. In ldlF cells, this difference is not significant. In heterokaryons composed of CHO cells, the restoration is high. In the heterokaryons composed of ldlF cells, it remains low. In heterokaryons composed of CHO and ldlF cells, the speed of the pore restoration is higher than in ldlF cells. (K) During intra-Golgi transport, co-localisation between PCI and GalT increases in control (CTR) cells but is inhibited in cells treated with AlF₄.