Figure 14.

Dynamics of pores during intra-Golgi transport and role of COPI in the generation of cisternal pores. (A,B) Before the release of the transport block, according to the CHM-15-CHM synchronisation protocol, the numerical density of the pores over all of the Golgi cisternae was high. (C,D) Disappearance of cisternal pores after the passage of VSVG. (F) High-pressure freezing. Human fibroblasts. Disappearance of cisternal pores after passage of PCI through the Golgi. (G) Addition of AlF₄ blocked restoration of the cisterna pores after the passage of VSVG. (H,J) Restoration of the numerical density of pores in the first medial cisterna after intra-Golgi transport and its inhibition for 25 min was blocked in heated ldlF cells. (see Methods). (I) Ministacks (3 h of nocodazole pre-treatment) after the passage of VSVG. There were no pores in the medial cisternae and no CMC and TMC. (J) Impairment of restoration of the cisternal pores in the ldlF cell heated to 40 °C for 2 min. Scale bars: 160 nm (A,D,G); 175 (B,H,I); 210 nm (C,E); 415 nm (F); 260 nm (J).