Table 2.
Reagents and algorithms.
| Reagent or Resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal antibody against GFP | Abcam (Cambridge, UK) | ab6556 |
| Rabbit polyclonal antibody against syntaxin 5 | Abcam (Cambridge, UK) | ab211417 |
| Rabbit polyclonal antibody against syntaxin 5 | Santa Cruz Biotechnology (USA) | Sc365124 |
| Rabbit polyclonal antibody against Bet1 | Abcam (Cambridge, UK) | ab42488 |
| Rabbit polyclonal antibody against GM130 | Abcam (Cambridge, UK) | ab31561 |
| Monoclonal antibody against VSVG (P5D4) | Abcam(Cambridge, UK) | ab50549 |
| Rabbit polyclonal antibody against mannosidase II | Abcam(Cambridge, UK) | ab12277 |
| Rabbit polyclonal against asialoglycoprotein receptor | Abcam(Cambridge, UK) | ab42488 |
| Rabbit polyclonal antibody against albumin | Abcam (Cambridge, UK) | ab42488 |
| Rabbit polyclonal antibody against Ykt6 | Abcam(Cambridge, UK) | ab236583 |
| Rabbit polyclonal anti-human antibody against β-1,3-galactosyltransferase 5 | MyBioSource Inc. (San Diego CA. USA) | MBS2026257 |
| PCI polyclonal against the α1 chain C-terminal domain of PCI | L. W. Fisher (National Institutes of Health) | N/A |
| Affinity-purified polyclonal sheep antibody against human pro-collagen I α1 | R&D Systems, Inc. (NY, USA) | AF6220 |
| Rabbit polyclonal antibody against ßCOP | Dr J. Lippincott-Schwartz (Howard Hughes Medical Institute, Ashburn, VA, USA) | N/A |
| Rabbit polyclonal antibody against GS15 | MyBioSource, Inc. (San Diego CA. USA) | MBS2525056) |
| Rabbit polyclonal antibody against membrin | Biorbyt Ltd. (NY; USA) | orb185573 |
| Rabbit polyclonal antibody against GOSR1(GS28) | Biorbyt Ltd. (NY; USA) | orb326297 |
| Rabbit polyclonal antibody against TGN46 | S Ponnambalam (Leeds University, UK) | N/A |
| GFP-albumin | Beznoussenko et al. [25] | N/A |
| Fab fragments of polyclonal antibodies conjugated with HRP | Jackson ImmunoResearch (West Grove, PA, USA) | Not produced anymore |
| Fab fragments of the polyclonal antibodies against IgGs | Jackson ImmunoResearch (West Grove, PA, USA) | Not produced anymore |
| Mouse IgG rabbit polyclonal antibody | ThermoFisher Scientific (USA; https://www.thermofisher.com, accessed on 15 March 2022) | 31188 |
| Goat anti-rabbit, anti-mouse, and anti-sheep IgG antibodies conjugated with Alexa 488, Alexa 546 | ThermoFisher Scientific (USA; https://www.thermofisher.com, accessed on 15 March 2022) | Many |
| Goat anti-rabbit Fab’ fragments of IgG conjugated with Nanogold | Nanoprobes (Yaphank, NY, USA) | #2004-1 mL |
| Protein A conjugated with gold particles of different sizes | Utrecht Universit. Utrecht; The Neverland) | Direct order |
| Chemicals, peptides, and recombinant proteins | ||
| Gold enhancer | Nanoprobes (Yaphank, NY, USA) | |
| Reagents for electron microscopy | EMS or Sigma (Merck) | Many |
| FUGENE6 transfection reagent | Roche (Monza, Italy). | |
| Recombinant DNA | ||
| Plasmid containing α1-chain of PC tagged with GFP | Beznoussenko et al. [25]; Patterson et al. [28] | N/A |
| Plasmid with GFP-Alb | Beznoussenko et al. [25] | N/A |
| Plasmid with PCIII-GFP | Perinetti et al. [112] | N/A |
| Software and algorithms | ||
| Image J | NIH, Bethesda | N/A |
| IMOD 4.0.11 package | The Boulder Laboratory for 3-D Electron Microscopy of Cells. University of Colorado Boulder | http://bio3d.colorado.edu/imod/accessed on 20 January 2020 |
| Prism: Version 9.4.2 package | GraphPad (NY; USA) |
https://www.graphpad.com/scientific-software/prism/ accessed on 20 January 2020 |