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Assessment of exosomal markers. (a) Western blot analysis of exosomal markers CD63 and CD9. Arrows indicate the bands corresponding to CD63 (53 kDa) and CD9 (28 kDa), respectively, C—control, CM—cerebral metastases. (b) Estimation of exosome levels in patient serum. CD63 protein levels in exosomes from breast cancer patients with primary breast cancer (PC) and breast cancer patients with cerebral (CM), viszeral (VM), and bone (BM) metastases were estimated using a CD63-specific ELISA.
