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. 2022 Mar 25;23(7):3567. doi: 10.3390/ijms23073567

Figure 5.

Figure 5

Inhibition of HIF-2α rescues zafirlukast-induced cell death. (A) mRNA expression levels of VEGF of the indicated cell lines treated with the HIF-2α inhibitor PT-2385 (20 µM) for 24 h were determined using qRT-PCR. Data are normalized to GAPDH expression and are presented as x-fold mRNA expression compared to control. Mean and SD of four independent experiments performed in triplicate are shown. *** p < 0.001; (B) 786-O and 293T (upper) and 786-O-EV and 786-O-VHL (lower) cells were pre-treated with the HIF-2α inhibitor PT-2385 (20 µM) for 24 h, followed by treatment with DMSO or 100 μM zafirlukast for 24 h, after which cell death was determined by quantifying PI uptake. Data are presented as percentage of PI-positive cells and mean and SD of three independent experiments performed in triplicate are shown. * p < 0.05, *** p < 0.001, n.s.: non-significant.