Figure 3.

ROS production and antioxidative enzyme activities in OR transgenic seedlings. (a) Nitrotetrazolium blue chloride (NBT) and 3,3′-diaminobenzidine (DAB) staining showed the production of O₂⁻ and H₂O₂ in the wild-type (WT) and OR-overexpressing (OE) and -silencing (or) seedlings under normal growth conditions (control) and drought treatment. (b,c) Enzymatic activity of superoxidase (SOD) (b) and catalase (CAT) (c) in different lines under normal growth conditions (control) and drought treatment. Data are means ± SEM (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, or better, Student’s t test). DW, dry weight.