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. 2022 Apr 1;23(7):3944. doi: 10.3390/ijms23073944

Figure 2.

Figure 2

NCTD induces apoptosis of bladder cancer cells under acidic environment. (A) EJ and UMUC3 cells were treated with an increasing concentration of NCTD (0–320 μM) for 24 h in physiological (pH 7.2–7.3) and acidic culture medium (pH 6.6–6.8). Cell viability was measured by the MTS assay. The logarithm of the NCTD concentration was taken, and then a curve was fitted to calculate the IC50 value. (B) EJ and UMUC3 cells were treated with NCTD (3 μM) in acidic medium, cell apoptosis was measured with Annexin-V/PI double-stained flow cytometry. Data are presented as the mean ± SD (n = 3), # p < 0.05 vs. the control group, * p < 0.05 vs. the acid group.