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. 2022 Apr 1;23(7):3936. doi: 10.3390/ijms23073936

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Antibody analysis of Sd^a surface expression in HEK293 cells transfected with B4GALNT2 constructs. One set of transfected cells were stained with human plasma containing anti-Sd^a (diluted in PBS) followed by staining with Alexa Fluor 647 conjugated Goat anti-human:IgM. The GFP positive population in HEK293 cells transfected with the vector CS-2719-M61 carrying (A) no construct (mock), (B) B4GALNT2 wt (GenBank accession number AJ517771), (C) B4GALNT2 with Sd(a−) associated SNPs rs7224888:T > C, (D) rs148441237:A > G or (E) rs61743617:C > T. Graphs present (F) the percentage of cells positive for the Alexa Fluor 647 signal and (G) the MFI of the Alexa Fluor 647 signal. Due to the small amounts of human anti-Sd^a available, only one set of transfected samples was run, not allowing for statistical evaluation.