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Analysis of Sda expression on erythrocyte surfaces by flow cytometry. Fixed erythrocytes were stained with human plasma containing anti-Sda (diluted in PBS) followed by PE-conjugated Goat anti-human:IgM. Erythrocytes from blood donors of (A) Sd(a–) phenotype (black line), also seen in the following graphs for comparison with other phenotypes. (B–D) Blue curve represents the common Sd(a+) phenotype (B) and Cad phenotypes, as shown by the Cad-a sample (C) and Cad-b (D).
