Skip to main content
. 2022 Apr 2;23(7):3980. doi: 10.3390/ijms23073980

Figure 4.

Figure 4

The effect short chain fatty acids on histone deacetylase (HDAC) activity (A) and the effect of HDAC inhibitors on CXCL10 release (B–E). HDAC activity of IECs after 24 h exposure to butyrate (2, 4 mM), propionate (4, 8 mM), acetate (4, 8 mM) or Trichostatin A (TSA) (10 μM) (A). Data are represented as mean ± SEM (n = 3). Significant differences are shown as * p < 0.05, **** p < 0.001 compared to control. CXCL10 release by intestinal epithelial cells after 24 h, activated with IFN-γ (B,C) or IFN-γ+TNF-α (D,E) and treated with 2 mM butyrate; 10 μM TSA; 1, 5, 15 μM tinostamustine (Tino); 10, 20, 50 μM TMP269 (TMP) (B,C) or with 2 mM butyrate; 10 μM TSA; 10, 50 μM droxinostat (Drox); 5, 20 μM RGFP966 (RGF); 1, 10 μM tacedinaline (Tace); 5, 20 μM Cay10683 (Cay) (D,E). 0.5% DMSO served as a control. Data are represented as mean ± SEM (n = 3). Significant differences are shown as * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.001 compared to IFN-γ-activated cells (B,D) or IFN-γ+TNF-α-activated cells (C,E).