Skip to main content
. 2022 Apr 2;23(7):3980. doi: 10.3390/ijms23073980

Figure 5.

Figure 5

The effect of butyrate and histone deacetylase (HDAC) inhibitor Trichostatin A (TSA) on mRNA expression of genes related to the STAT1 signaling cascade. Intestinal epithelial cells were activated with IFN-γ or IFN-γ+TNF-α and treated with 2 mM butyrate (green bars) or 10 μM TSA (pink bars) for 4 h. mRNA expression was measured in CXCL10 (A,B), IRF9 (C,D) JAK2 (E,F) and SOCS1 (G,H). Data are represented as mean ± SEM (n = 4). Significant differences are shown as ** p < 0.01, *** p < 0.001, **** p < 0.001 Control compared to butyrate, TSA, IFN-γ (A,C,E,G) or IFNγ+TNFα (B,D,F,H) activated cells and activated cells compared to butyrate or TSA treated activated cells.