Table 1.

Parameters that were used in the GLOBLE model for prediction of the impact of CTCF depletion. The linear-quadratic parameters α and β and the corresponding lethalities ε_i and ε_c were determined from the survival curves under GFP KD conditions, after pooling all biological replicates. The genome size of Hela Kyoto was taken from [60] and that of U2OS estimated from that value multiplied by the genome size ratio in Figure 1b. The genome size for mouse mESC-AID-CTCF cells was taken from [63] (see Section 2.6). The default domain size was chosen to be identical for all cell lines.

Cell Line	HeLa Kyoto	U2OS	mESC-AID-CTCF
Genome size [Mbp]	9.7 × 103	11.93 × 103	5.2× 103
Domain size [Mbp]	2	2	2
α [Gy−1]	0.145± 0.014	0.179 ± 0.034	0.111 ± 0.025
β [Gy−2]	0.012± 0.002	0.067 ± 0.0067	0.013 ± 0.0029
α/β [Gy]	12.1	2.67	8.54
εi	0.0030	0.0030	0.0043
εc	0.0555	0.2310	0.1085