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. Author manuscript; available in PMC: 2022 Apr 11.
Published in final edited form as: Nature. 2021 Nov 10;600(7888):302–307. doi: 10.1038/s41586-021-04083-0

Extended Figure 6. Genetic study of B. fragilis Bcat orthologue (BF9343–3671).

Extended Figure 6.

(A) Confirmation of the target gene deletion by PCR. (B) The knockout strain (BF9343-Δ3671) shows comparable growth pattern to isogenic WT strain (grown in duplicate per group), and a complemented strain of KO strain with empty vector shows same pattern to BF9343–3671 complemented strain. (C) BF9343-Δ3671 complementation can recover the production of di-branched C17/C17 BfaGC production to wild-type level. (D) WT and mutant strain (N=5 for each group) can colonize mouse in comparable density. All results represent of two independent experiments with similar trend. For gel source data, see Supplementary Figure 1.