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. 2022 Apr 2;23(7):3961. doi: 10.3390/ijms23073961

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression levels of CmCHS at different times after dsRNA injection. The CmCHS expression levels in the third-instar C. medinalis larvae were detected with ddPCR four days after injection of dsCmCHS or the mixture of dsCmdsRNase and dsCmCHS. Larvae injected with dsGFP were used as the control group. Each bar represents the mean ± SD. One asterisk above bars indicates a significant difference at p < 0.05 and two asterisks indicate a very significant difference at p < 0.01 according to Duncan’s test.