Table 2.
Primers used for cloning and expression analysis of CmdsRNase from C. medinalis.
| Primer Name | Primer Sequence (5′→3′) | Primer Usage |
|---|---|---|
| CmdsRNase-F | ATGCATTCGCTGGTGCTTC | RT-PCR |
| CmdsRNase-R | TTAGGACAGAAGACCAACAAC | |
| CmdsRNase-dF | GACGCCAAGTGCCAGTTCCT | ddPCR |
| CmdsRNase-dR | GTGCTTCAGCCGCCGTATAGT | |
| CmCHS-dF | TGGAATACCTTCGCCAGTCATC | |
| CmCHS-dR | CCAGGAACACCAGGAGGCATT | |
| CmdsRNase-iF | CGACAGGAATCGTCTTGAAG | dsRNA synthesis |
| CmdsRNase-iR | AGGCTATACGAGCACGGAGGT | |
| CmdsRNase-dsF | taatacgactcactatagggCGACAGGAATCGTCTTGAAG | |
| CmdsRNase-dsR | taatacgactcactatagggAGGCTATACGAGCACGGAGGT | |
| CmCHS-iF | ACGAGGTTACACGAGAGG | |
| CmCHS-iR | CATCCAATGTTCCAATGTTCCT | |
| CmCHS-dsF | taatacgactcactatagggACGAGGTTACACGAGAGG | |
| CmCHS-dsR | taatacgactcactatagggCATCCAATGTTCCAATGTTCCT | |
| GFP-iF | GCCAACACTTGTCACTACTT | |
| GFP-iR | GGAGTATTTTGTTGATAATGGTCTG | |
| GFP-dsF | taatacgactcactatagggGCCAACACTTGTCACTACTT | |
| GFP-dsR | taatacgactcactatagggGGAGTATTTTGTTGATAATGGTCTG |
Note: The lowercase letters in the primer sequences represent the sequence of the T7 promoter.