Figure 2.

Southern blot analysis and phenotype of Micro-tom plants. (A) Southern blot confirming production of homoplastomic tpMicro-tom plants. Ten micrograms of total DNA was digested using Mfe I restriction enzyme, and the DNA fragments were separated on a 0.8% agarose gel for blotting. Lane 1 = Dig-labelled DNA ladder III (Roche); Lane 2 = WT Micro-tom (1298 bp); Lane 3 = tpMicro-tom (3353 bp). (B) Phenotype comparison of 8-week-old WT Micro-tom and T₁ tpMicro-tom plants. The seeds were first germinated on Murashige and Skoog (MS) medium, and the plants were transplanted to soil after 3 weeks for further growth in a greenhouse.