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Total cell-associated uronic acids from S. marcescens strains cultured in M9 medium supplemented with glucose were quantified by colorimetric assay. Uronic acid levels were normalized to culture density and calculated based on a glucuronic acid standard curve. Significant differences relative to the acapsular strain ATCC 13880 were assessed by one-way ANOVA with Dunnett’s multiple comparisons test (****, P<0.0001).
