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. 2022 Mar 30;18(3):e1010423. doi: 10.1371/journal.ppat.1010423

Table 1. S. marcescens strains and recombinant plasmids used in this study.

Name Description Reference
S. marcescens
ATCC 13880 pond water isolate American Type Culture Collection
gn773 human bloodstream infection isolate, also referred to as 2880STDY5682865 [11]
UMH7 human bloodstream infection isolate [16]
UMH9 human bloodstream infection isolate [16]
UMH11 human bloodstream infection isolate [16]
UMH9 Δwzi::nptII 1392-bp deletion of the wzi ORF and insertion of nptII from pKD4 this study
UMH9 ΔCPSv::nptII 16,214-bp deletion of the capsule variable region and insertion of nptII from pKD4 this study
UMH9 ΔneuB::nptII ΔneuB deletion allele retaining only the neuB start and stop codons with nptII from pKD4 inserted this study
UMH9 ΔneuAB in-frame ΔneuAB deletion retaining the first 30 bp of neuA and the last 30 bp of neuB this study
plasmids
pTOX11nptII pTOX11 derivative encoding kanamycin resistance this study
pBAD2 pBAD18-Km harboring the UMH9 neuA and neuB ORFs this study
pBB16 pBBR1MCS-5 harboring the UMH9 wzi ORF this study
pBB23 pBBR1MCS-5 harboring the UMH9 neuB ORF this study