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. 2022 Apr 2;23(7):3966. doi: 10.3390/ijms23073966

Figure 4.

Figure 4

Analysis of the interaction between curcumin derivatives with recombinant EGFR and NF-κB by microscale thermophoresis (MST). The recombinant proteins were used at a concentration of 200 nM, while the concentration of curcumin, N-(3-nitrophenylpyrazole) curcumin, and the curcumin derivative 1A9 ranged from 100 to 100,000 nM. The migration of the fluorescent proteins was determined upon local heating using a Monolith NT.115Pico with 40% LED power and 80% MST power for EGFR and with 20% LED power and 20% MST power for NF-κB at room temperature.