Table 7.

Diarrheal/laxative activity of EAC.

Samples	Dose g/kg mL/kg (PO)	First Stool/Latency Time (Minutes)	Total Number of Stools	Number of Wet Stools	Weight of Stool (Grams)	Percent Wet Stool (%)
EAC + DW	1	23.25 ± 2.50	14.75 ± 3.59	09 ± 2.16	10.05 ± 2.87	63.03 ± 13.97
	2	21.5 ± 1.73	15.75 ± 1.71	12 ± 1.63	11.33 ± 3.21	77.64 ± 18.26
	3	19.75 ± 2.22	16 ± 2.45	13 ± 2.94	13 ± 2.45	80.55 ± 7.97
Loperamide hydrochloride (4 mg/kg) was administered (PO) 30 min before fractions/distilled water/castor oil.
Distilled water (-ve control)	6	70.33 ± 2.52	8.33 ± 2.52	0.00 ± 0.00	7.50 ± 2.29	0.00 ± 0.00
Castor oil (+ ve control)	6	17.33 ± 2.52 ***	16.67 ± 2.08 **	14.33 ± 1.53 ***	15.07 ± 1.27 ***	89.74 ± 2.78 ***
EAC + Lopr	1	41.33 ± 3.21 ***	11.00 ± 2.65	2.00 ± 1.00	8.43 ± 1.81	20.16 ± 13.10 ***
	2	40.33 ± 1.53 ***	11.67 ± 3.06	5.00 ± 1.00 *	9.23 ± 1.00	43.30 ± 2.90 ***
	3	30.33 ± 1.53 ***	9.67 ± 1.53	6.00 ± 1.00 **	9.20 ± 1.05	62.04 ± 1.86 ***

Data are represented as mean ± SEM. The data were analyzed by one-way ANOVA followed by Dunnett’s test using GraphPad prism version 6.01; p ≤ 0.05 was considered as significant (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001). EAC, ethyl acetate fraction of Chrozophora tinctoria; Lopr, loperamide hydrochloride; DW, distilled water, PO, per orally/by orally.