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. 2022 Mar 29;27(7):2218. doi: 10.3390/molecules27072218

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of AME stimulation on the expression of sex-related genes in zebrafish: (A) female, lhcgr in ovaries; (B) female, cyp19a1a in the brain; (C) male, ar in the testes; (D) male, cyp19a1b in the brain. Five adult zebrafish were used for each experimental group at each sampling time. Statistical differences in gene expression between the AME-supplemented-diet group and the vehicle group were tested using one-way ANOVA, and levels of significance are denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001.