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. 2022 Mar 25;27(7):2109. doi: 10.3390/molecules27072109

Figure 6.

Figure 6

3-Hydroxyterphenyllin (3-HT) and candidusin A (CDA) attenuate reactive oxygen species (ROS) generation induced by palmitic acid (PA) in human podocytes. The level of ROS formation was measured by using the 2′,7′-dichlorofluorescin diacetate (DCFDA) fluorescent dye. Podocytes were seeded on 96-well black plates for 24 h before treatment with 600 µM PA and 3-HT or CDA at 50 µM, plus a vehicle (control) for another 24 h. N-acetylcysteine (NAC) (10 mM) was used as the positive control. The data are expressed as the mean intensity of DCFDA fluorescence/Hoechst ± standard error of the mean (n = 5). * p < 0.05 compared with the control group; ## p < 0.01 compared with the PA-treated group.