KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PAX7 | DSHB | RRID:AB_528428 |
| MyoD | Santa Cruz | Cat# sc-760, RRID:AB_2148870 |
| Myogenin | DSHB | Cat# F5D, RRID:AB_2146602 |
| Anti-human dystrophin | Millipore | MABT827 |
| GFP Polyclonal Antibody | Invitrogen | Cat# A10262, RRID:AB_2534023 |
| Dystrophin | Abcam | Cat# 12577 |
| Lamin A/C Monoclonal Antibody | Thermo Fisher | Cat# MA3-1000, RRID:AB_325377 |
| Anti-Laminin antibody | Sigma | Cat# L9393, RRID:AB_477163 |
| Anti-human Laminin antibody | DSHB | Cat# 2E8, RRID:AB_528343 |
| Desmin Antibody | DAKO | Cat# M0760, RRID:AB_2335684 |
| ERTR-7 Antibody | Santa Cruz | Cat# sc-73355 RRID:AB 1122890 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| CHIR99021 | Cayman | Cat# 13122 |
| DAPT | Cayman | Cat# 13197 |
| FGF-2 | Pepro Tech | Cat# 100-18B |
| FGF8 | Peprotech | Cat# 100-25 |
| BaCl2 | Sigma | Cat# 342920 |
| Y-27632 | Tocris | Cat# 1524 |
| Hoechst 33342 | Invitrogen | Cat# H3570 |
| Pyronin-Y | Thermo Scientific | Cat# J61068.09 |
| Critical commercial assays | ||
| SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase | Invitorgen | Cat# 12574018 |
| KAPA SYBR® FAST qPCR Master Mix (2X) Kit |
Kapabiosystems | Cat# KR0389 |
| Phire Tissue Direct PCR Master Mix | Thermo Scientific | Cat# F170S |
| Deposited data | ||
| SC RNA-seq data | GEO | GSE165075 |
| Code for SC RNA-seq data analysis | Github | https://github.com/skannan4/satellite-cell-transplantation |
| Bulk RNA-seq data | GEO | GSE190233 |
| Experimental models: cell lines | ||
| Human H9 ESC | WiCell | Cat# WA09, RRID:CVCL_9773 |
| Human primary myoblasts | N/A | |
| Gibco CF1 Mouse Embryonic Fibroblasts, Irradiated cell line | Gibco | Cat# A34181 RRID:CVCL_RB05 |
| Experimental models: organisms/strains | ||
| NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | JAX | Cat# JAX:005557, RRID:IMSR_JAX:005557 |
| NSG-mdx4Cv mice | (Arpke et al., 2013) | N/A |
| Oligonucleotides | ||
| Primer: eGFP Forward- ACGTAAACGGCCACAAGT, Reverse- AAGTCGTGCTGCTTCATGTG | This paper | |
| Primer: huPmp22 forward- ACATAGGGAAGGGAGGAAGG, reverse- TTGGTTTGGTTTGAGTTTGG | This paper | |
| Primer: huCalC forward- ACACCGGCTGGAGTATTGTC, reverse- AAAATCCCCAGGGAAATCAC | This paper | |
| Primer: huPAX7 forward- CTGGCTACCAGTACGACCAGT, reverse- GCACGCCGGTTACTGAAC | This paper | |
| Recombinant DNA | ||
| Software and algorithms | ||
| Imaris | Oxford Instrument | |
| FlowJo | FlowJo Software | RRID:SCR_008520; https://www.flowjo.com |
| GraphPad Prism | GraphPad Software Inc. | RRID:SCR_002798; https://www.graphpad.com/scientific-software/prism/ |
| Fiji | https://doi.org/10.1038/nmeth.2019 | RRID:SCR_002285; https://imagej.net/Fiji |
| R | https://www.r-project.org/ | |
| Other | ||
| Quick-RNA Microprep Kit | Zymo Research | Cat# R1050 |