Table 1.
Identification and content of bufadienolides in Kalanchoe daigremontiana water extract using UHPLC-QTOF-MS analysis.
| No. | Identification | RT [min] |
Meas. m/z [M + H] |
Ion Formula | mσ | Content [ng/mg d. w.] * |
|---|---|---|---|---|---|---|
| 1 | tetrahydroxy-bufadienolide-rhamnoside | 2.06 | 581.2954 | C30H45O11 | 17.2 | 107.2 ± 9.1 |
| 2 | tetrahydroxy-bufadienolide-dHex isomer 1 | 2.27 | 581.2953 | C30H45O11 | 9.3 | <LLOD |
| 3 | tetrahydroxy-oxo-bufadienolide-acetate isomer 1 | 2.71 | 491.2276 | C26H35O9 | 7.1 | 186.8 ± 16.1 |
| 4 | tetrahydroxy-bufadienolide-dHex isomer 2 | 2.94 | 581.2955 | C30H45O11 | 3.7 | <LLOD |
| 5 | bersaldegenin-acetate/bryophyllin-C isomer 1 | 3.68 | 475.2327 | C26H35O8 | 13.7 | 35.6 ± 10.5 |
| 6 | bryophyllin-B/bryotoxin-B isomer 1 | 4.81 | 489.2119 | C26H33O9 | 19.2 | 113.4 ± 12.3 |
| 7 | daigremontianin isomer 1 | 4.84 | 487.1965 | C26H31O9 | 329.8 | 56.2 ± 8.2 |
| 8 | bryophyllin-A/bryotoxin-C isomer 1 | 4.91 | 473.2174 | C26H33O8 | 5.3 | 573.5 ± 27.2 |
| 9 | bryophyllin-B/bryotoxin-B isomer 2 | 5.05 | 489.2122 | C26H33O9 | 10.5 | <LLOD |
| 10 | daigremontianin isomer 2 | 5.44 | 487.1967 | C26H31O9 | 14.8 | 81.4 ± 4.5 |
| 11 | bryophyllin-A/bryotoxin-C isomer 2 | 5.73 | 473.2172 | C26H33O8 | 6.1 | <LLOD |
| 12 | tetrahydroxy-oxo-bufadienolide-acetate isomer 2 | 6.06 | 477.2483 | C26H37O8 | 13.3 | <LLOD |
| 13 | daigremontianin isomer 3 | 6.23 | 487.1967 | C26H31O9 | 8.8 | <LLOD |
| 14 | bryophyllin-A/bryotoxin-C isomer 2 | 6.28 | 473.2175 | C26H33O8 | 31.4 | <LLOD |
| 15 | bersaldegenin-acetate/bryophyllin-C isomer 2 | 6.85 | 475.2332 | C26H35O8 | 3.4 | 19.2 ± 3.6 |
| 16 | daigredorigenin-acetate | 8.20 | 461.2537 | C26H37O7 | 7.5 | 2.0 ± 0.5 |
| 17 | daigremontianin | 8.32 | 487.1964 | C26H31O9 | 2.6 | 399.4 ± 19.3 |
| 18 | methyl-daigremonate isomer 1 | 10.43 | 503.2277 | C27H35O9 | 3.0 | <LLOD |
| 19 | methyl-daigremonate isomer 2 | 11.94 | 503.2281 | C27H35O9 | 11.2 | <LLOD |
| 20 | bersaldegenin-1,3,5-orthoacetate | 13.86 | 457.2224 | C26H33O7 | 6.8 | 757.4 ± 18.7 |
|
Total content 2332.1 ± 30 | ||||||
* Average values of bufadienolide content obtained from three repetitions of the experiment. Note: d.w.—dry weight; dHex—deoxyhexose; < LLOD—below the lower limit of detection.