Table 4.
Primers sequence used and their sequences.
| Primer | Forward Primer (5′–3′) | Reverse Primer (5′–3′) | Size | Accession No. |
|---|---|---|---|---|
| PCNA | ATCTAGACGTCGCAACTCCG | GCTGCACTAAGGAGACGTGA | 173 | NM_022381.3 |
| Mmp9 | GATCCCCAGAGCGTTACTCG | GTTGTGGAAACTCACACGCC | 132 | NM_031055.2 |
| Collagen | GCAATGCTGAATCGTCCCAC | CAGCACAGGCCCTCAAAAAC | 176 | NM_053304.1 |
| Fibronectin | GGATCCCCTCCCAGAGAAGT | GGGTGTGGAAGGGTAACCAG | 188 | NM_019143.2 |
| NF-κβ1 | CCACTGTCAACAGATGGCCC | CTTTGCAGGCCCCACATAGT | 177 | NM_001276711.1 |
| TNF-α | GGCTTTCGGAACTCACTGGA | GGGAACAGTCTGGGAAGCTC | 164 | NM_012675.3 |
| IL-10 | GCTCAGCACTGCTATGTTGC | TTGTCACCCCGGATGGAATG | 76 | NM_012854.2 |
| IL-8 | ACAGGCAGGCTGTAGTTGTC | ATCACCAGCGAGTTTCCCAG | 70 | NM_019310.1 |
| IL-4 | CGTGATGTACCTCCGTGCTT | GTGAGTTCAGACCGCTGACA | 88 | NM_201270.1 |
| IL-1β | GAGTCTGCACAGTTCCCCAA | TCCTGGGGAAGGCATTAGGA | 158 | NM_031512.2 |
| MCP-1 | TAGCATCCACGTGCTGTCTC | CAGCCGACTCATTGGGATCA | 94 | NM_031530.1 |
| TGF-β1 | AGGGCTACCATGCCAACTTC | CCACGTAGTAGACGATGGGC | 168 | NM_021578.2 |
| IL-1β | GAGTCTGCACAGTTCCCCAA | TCCTGGGGAAGGCATTAGGA | 158 | NM_031512.2 |
| Gapdh | GCATCTTCTTGTGCAGTGCC | GGTAACCAGGCGTCCGATAC | 91 | NM_017008.4 |
| GLP1 | CACCTCCTCTCAGCTCAGTC | CGTTCTCCTCCGTGTCTTGA | 128 | NM_012707.2 |
| GLPr1 | CTTGGAGACATAGAAGGGGGAC | AGGAGCATGCCTCTGGGTAG | 128 | NM_172091.2 |
| EGF-β1r | GGCATCATGGGGGAGAACAA | GGATCTTTGGCCCATAGGTACAG | 100 | NM_001393707.1 |
| EGF-β1 | GGTCCACCCATTGGCAAAAC | CACGAATCCTTCCCGACACA | 118 | NM_012842.2 |
| PPAR-α | GTCCTCTGGTTGTCCCCTTG | GTCAGTTCACAGGGAAGGCA | 176 | NM_013196.2 |
| PGC-1α | TTCAGGAGCTGGATGGCTTG | GGGCAGCACACTCTATGTCA | 70 | NM_031347.1 |
| FGF | GAGCGACCCTCACATCAA | CGTTTCAGTGCCACATACC | 222 | NM_019305.2 |
| VEGF | GATCCAGTACCCGAGCAGTCA | TCTCCTTTCTTTTTGGTCTGCAT | 83 | NM_053549.1 |
| Bax | CGAATTGGCGATGAACTGGA | CAAACATGTCAGCTGCCACAC | 109 | NM_017059.2 |
| Bcl-2 | GACTGAGTACCTGAACCGGCATC | CTGAGCAGCGTCTTCAGAGACA | 135 | NM_016993.1 |
| Fas | GAGCGTTCGTGAAACCGACA | AGGTTGGTGCACCTCCACTTG | 128 | NM_139194.2 |
| FasL | CACCAACCACAGCCTTAGAGTATCA | CACTCCAGAGATCAAAGCAGTTCC | 172 | NM_012908.1 |
| P38 | CGGCTTGCTCATGTCCTCAGAAC | GGAGGGCGGCTGCACATACAC | 214 | NM_031020.2 |
| P53 | CATGAGCGTTGCTCTGATGGT | GATTTCCTTCCACCCGGATAA | 67 | NM_030989.3 |
| Casp-3 | GAGACAGACAGTGGAACTGACGATG | GGCGCAAAGTGACTGGATGA | 147 | NM_012922.2 |
| 16s rRNA | AGAGTTTGATCCTGGCTCAG | CTACGGCTACCTTGTTACGA | 1507 | NR_043997.1 |