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. 2022 Mar 28;27(7):2185. doi: 10.3390/molecules27072185

Figure 2.

Figure 2

Various mechanistic assays performed on human lung cancer cell line A-549. I. Nuclear morphological changes as indicated by arrow using DAPI staining with chloroform fraction of bark treatment. (A) Control (B) 50 µg/mL (C) 75 µg/mL and (D) 100 µg/mL II. Effect of chloroform fraction on A-549 in the production of reactive oxygen species evaluated by oxidation of DCFDA by hydrogen peroxide through fluoroscence microscopy at 24 h posttreatment. (A) Control (B) H2O2 0.5% (C) 50 µg/mL (D) 75 µg/mL (E) 100 µg/mL III. In vitro colony formation assay of bark chloroform fraction (A) Control (B) 50 µg/mL (C) 75 µg/mL and (D) 100 µg/mL IV. In vitro wound healing assay chloroform fraction. 0 h (A) Control (B) 50 µg/mL (C) 75 µg/mL and (D) 100 µg/mL. After 24 h (E) Control (F) 50 µg/mL (G) 75 µg/mL and (H) 100 µg/mL.