Figure 2.

Effects of complex C1 on pancreatic carcinoma cells viability and potential to form colonies. (A) MIA-PaCa-2 cells were treated with DMSO as a vehicle, with various concentrations of initial compound, AHC and complex C1 for 24 h. The cell’s viability was calculated in relation to DMSO treated cell’s viability, which was set as 100%. Results were presented as the means ± SEM (standard error mean) of at least three independent experiments. Student’s t-test was used to calculate p-values (* p ≤ 0.05). (B) PANC-1 cells were treated with DMSO as a vehicle, various concentrations of initial compound, AHC and complex C1 for 24 h. The cell’s viability was calculated in relation to DMSO treated cell’s viability, which was set as 100%. Results were presented as the means ± SEM (standard error mean) of at least three independent experiments. Student’s t-test was used to calculate p-values (* p ≤ 0.05). (C) PANC-1 cells were treated with DMSO as a vehicle and with lower concentrations of complex C1 for 24 h, 48 h and 72 h. (D) Colony formation assay was performed on PANC-1 cells treated with DMSO as a vehicle or with 0.5 µM of complex C1 for a period of 10 days. Representative macroscopic images of colonies in Petri dish upon treatments are presented with corresponding microscopic images of colonies as an example at bottom right corners. Images were captured by digital camera. (E) Relative number of PANC-1 colonies and relative colony area were calculated in relation to these parameters measured in DMSO treated cells that were set as 100%. Results were presented as the means ± SEM of at least three independent experiments. Student’s t-test was used to calculate p-values (* p ≤ 0.05).