Table 2.

The study inclusion and exclusion criteria for clinical study.

Inclusion criteria

(1) Female subjects with wrinkle aged from 50 to 65 (2) Healthy person who does not have acute or chronic physical disease including skin disease (3) Signed and informed consent; the purpose and the protocol of the study were explained to subjects (4) Subjects cooperative and available for follow-up during the study period

Exclusion criteria

(1) Pregnancy or nursing condition or planning to become pregnant (2) Treatment of immune-suppression within one month (3) Participation in a previous study without an appropriate intervening period (three months) between studies (4) Sensitivity or hypersensitivity skin (5) Damaged skin in, or around the test site, which includes sunburn, tattoos, scars or other disfiguration on the test site (6) Use of similar treatment during the previous three months (7) Having an experience on examination site (skin decortications, Botox and other skin treatment) (8) Chronic disease (diabetes, asthma, high blood-pressure) (9) In case of atopic dermatitis (10) Any difficult which may interfere with the aim of the study as the judgment of the investigator