Appendix 1—figure 1. Biochemical characterization of the Gloeobacter PSI trimer.

(A) SDS-PAGE analysis of the PSI trimer. Protein bands were identified by mass spectrometry. It should be noted that some of the subunits in the PSI trimer were not detected by SDS-PAGE, probably due to their poor staining by CBB, but were visualized by the present cryo-EM map. (B) HPLC analysis of pigments extracted from the PSI trimer monitored at 440 nm. Letters of a and β-Car indicate Chl a and β-carotene, respectively. Data are representative of three independent experiments.