Figure 2.

(a and b) Lesion size progression during topical berberine-β-glycerophosphate (BER-GP) cream treatment (control, filled circles; vehicle, open circles; and BER-GP cream, filled squares). (c) Representative images of HE-stained skin sections obtained from a control mouse (infected and non-treated, left) and BER-GP cream treated mice (right). Scale bars: 800 μm. (d) Parasite burden in skin lesions and LNs of L. major-infected BALB/c mice after 35 days of topical treatment with BER-GP cream (15 mg/kg daily in two doses, filled squares), compared with non-treated mice (C = control, filled circles). Results are expressed as median (n = 6–11 per group). Data were analysed by a non-parametric Mann–Whitney test. * P < 0.05, ** P < 0.01, *** P < 0.001. This figure appears in colour in the online version of JAC and in black and white in the print version of JAC.