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. 2022 Jan 28;77(4):1072–1081. doi: 10.1093/jac/dkac007

Figure 3.

Figure 3.

(a) Immunohistochemical analysis in skin lesions of non-treated L. major-infected BALB/c mice (C = control), or after 35 days of topical treatment with berberine-β-glycerophosphate (BER-GP) cream for neutrophils (NIMP-14, light grey), macrophages (F4/80, dark grey) and lymphocytes (CD3, white). Boxes represent median (central line) ± 95% CI (upper and lower edges) and whiskers represent minimum and maximum values (n = 5 per group). (b) Representative images of skin sections for control (infected untreated mice, top) and mice treated with BER-GP cream (bottom) stained with antibodies against NIMP-R14 (neutrophils) and F4/80 (macrophages). Scale bars: 600 μm. (c) Cytokine expression in skin lesions from L. major-infected BALB/c mice after 35 days of topical treatment with BER-GP cream, compared with non-treated mice (C = control), expressed as fold change. Boxes represent median (central line) ± 95% CI (upper and lower edges) and whiskers represent minimum and maximum values (n = 5 per group). Data were analysed by a non-parametric Mann–Whitney test. * P < 0.05, ** P < 0.01. This figure appears in colour in the online version of JAC and in black and white in the print version of JAC.