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. 2022 Mar 31;11:e71469. doi: 10.7554/eLife.71469

Figure 8. R7 photoreceptors have identical targeting defects in Lar mutants and Lar/sns transheterozygotes.

(A–F) Single optical slices of adult optic lobes (OLs) showing R7 and R8 photoreceptors labeled for Chaoptin (24B10, red). R7 photoreceptor axons end in the M6 layer of the medulla (outlined in white), while R8 axons end in M3 layer (arrowheads). (A–C) Heterozygote controls showing normal R7 targeting in the M6 layer. (D–F) Lar/sns transheterozygotes and Lar mutants, showing abnormal R7 targeting, with most R7 axons retracting to the M3 layer. (G) Quantification of R7 axon retractions in control and mutant animals. R7 axons were counted in at least 10 optical slices per OL. Each data point is the average of 10–12 optical slices per OL. The data were analyzed using one-way ANOVA followed by Tukey’s post-hoc correction. ****p<0.0001. (H–J) Single optical slices showing the morphology of R7 terminals in Lar451/+ control, Lar451/snsxb3 transheterozygotes, and Lar mutants. Control animals show normal rounded bouton-shaped R7 terminals (H, arrow). Some R7 terminals that do not retract and stay in the M6 layer have abnormal R7 terminal morphologies, with thin and spear-shaped terminals (I, J, arrows). Scale bar, 20 µm.

Figure 8.

Figure 8—figure supplement 1. Abnormal R7 projection examples upon pan-neuronal Sns RNAi knockdown.

Figure 8—figure supplement 1.

(A–D) Single optical slices of adult optic lobes (OLs) from four different animals showing R7 and R8 photoreceptors labeled for Chaoptin (24B10, red) upon neuronal Sns RNAi knockdown. A small number of R7 axon retractions are seen upon Sns RNAi knockdown (asterisks). Some R7 axons abnormally innervate neighboring R7 columns (arrows).