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. 2022 Mar 31;11:e71469. doi: 10.7554/eLife.71469

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (Drosophila melanogaster) Canton S (CS) Bloomington Drosophila Stock Center (BDSC) BDSC #64349
Genetic reagent (D. melanogaster) Tubulin-GAL4 BDSC BDSC #5138
Genetic reagent (D. melanogaster) elavC155-GAL4 BDSC BDSC #458
Genetic reagent (D. melanogaster) OK107-GAL4 BDSC BDSC #854
Genetic reagent (D. melanogaster) UAS-2xEGFP BDSC BDSC #6874
Genetic reagent (D. melanogaster) UAS-mCD8-GFP BDSC BDSC #32185
Genetic reagent (D. melanogaster) snsMIMIC-MI03001 BDSC BDSC #35916
Genetic reagent (D. melanogaster) larMIMIC-MI02154 BDSC BDSC #35972
Genetic reagent (D. melanogaster) snsEY08142 BDSC BDSC #17434
Genetic reagent (D. melanogaster) Sns-T2A-GAL4 This paper Lab of Dr. Kai Zinn
Genetic reagent (D. melanogaster) Lar-T2A-GAL4 This paper Lab of Dr. Kai Zinn
Genetic reagent (D. melanogaster) UAS-sns-RNAi Vienna Drosophila Resource Center (VDRC) VDRC #109442
Genetic reagent (D. melanogaster) UAS-sns-RNAi VDRC VDRC #877
Genetic reagent (D. melanogaster) UAS-lar-RNAi Developmental Studies Hybridoma Bank (DSHB) DSHB #40938
Genetic reagent (D. melanogaster) UAS-lar-RNAi DSHB DSHB #34965
Genetic reagent (D. melanogaster) UAS-kirre-RNAi DSHB DSHB #67340
Genetic reagent (D. melanogaster) snsxb3 PMID #10859168 Gift of Susan Abmyer
Genetic reagent (D. melanogaster) snsDf DSHB DSHB #23165
Genetic reagent (D. melanogaster) lar13.2 PMID #8598047 DSHB #8774
Genetic reagent (D. melanogaster) lar451 PMID #11683994
Genetic reagent (D. melanogaster) lar2127 PMID# 11683993
Antibody Rabbit anti-GFP (rabbit polyclonal) Thermo Fisher #A11122 (1:500)
Antibody Rabbit anti-RFP (rabbit polyclonal) Rockland Inc. #600-401-379 (1:500)
Antibody Mouse anti-Dlg (mouse monoclonal) DSHB #4F3 (1:100)
Antibody Mouse anti-Bruchpilot (mouse monoclonal) DSHB #nc82 (1:10)
Antibody Mouse anti-Chaoptin (mouse monoclonal) DSHB #24B10 (1:10)
Antibody Mouse anti-FasII (mouse monoclonal) DSHB #1D4 (1:3)
Antibody Mouse anti-Trio (mouse monoclonal) DSHB #9.4A (1:20)
Antibody Mouse anti-Lar (mouse monoclonal) DSHB #9D8 (1:3)
Antibody Mouse anti-Repo (mouse monoclonal) DSHB #8D12 (1:10)
Antibody Mouse anti-Evenskipped (mouse monoclonal) DSHB #3C10 (1:10)
Antibody Mouse anti-human IgG (Fc specific) (mouse monoclonal) Bio-Rad #MCA647G (1:200)
Antibody Rabbit anti-alkaline phosphatase (AP) (rabbit polyclonal) AbD Serotec (1:1000)
Antibody Mouse anti-AP:biotin (mouse monoclonal) eBioscience, Thermo Fisher #13-9870-82 (1:200)
Antibody Goat anti-HRP-Alexa 488 (goat polyclonal) Jackson ImmunoResearch #123-545-021 (1:50)
Antibody Goat anti-HRP-Alexa 594 (goat polyclonal) Jackson ImmunoResearch #123-585-021 (1:50)
Antibody Goat anti-mouse-Alexa 488 (goat polyclonal) Thermo Fisher #A110029 (1:500)
Antibody Goat anti-mouse-Alexa 568 (goat polyclonal) Thermo Fisher #A11031 (1:500)
Antibody Goat anti-rabbit-Alexa 488 (goat polyclonal) Thermo Fisher #A11008 (1:500)
Antibody Goat anti-rabbit-Alexa 568 (goat polyclonal) Thermo Fisher #A11036 (1:500)
Antibody Goat anti-mouse-AP (goat polyclonal) Jackson ImmunoResearch #115-055-003 (1:200)
Peptide, recombinant protein Streptavidin:HRP Thermo Fisher #N100 (1:500)
Commercial assay or kit Ultra-TMB Thermo Fisher #34028
Commercial assay or kit BluePhos  Seracare #5120-0059