Figure 3.

Enrichment methods applied to site-specific phosphorylation data. The differential enrichment score from two separate enrichment approaches is shown. On the left, significant pathways by differential correlation analysis are shown, and on the right, the same pathways showing an enrichment comparison between substrate phosphosite abundance are shown. Enrichment in the short surviving patient set is depicted in black bars, in the positive direction, and enrichment in long surviving patient set is depicted in gray bars, in the negative direction. Sets of known substrates for the kinases were derived from the Phosphosite Plus database. For demonstration purposes, significance of the correlation results was not corrected for multiple hypotheses. Comparing the two plots shows that although the kinase substrates are all significantly more abundant in the short surviving cohort, the long surviving cohort had a greater degree of correlation between those substrates—suggesting more controlled regulation.