FIGURE 3.

Conditional knockout of p120 suppressed epidermal differentiation. The skin was removed from the mice and sections of paraffin-embedded epidermis were processed for H&E and immunohistochemical staining. The antibodies used in the immunochemical staining included K1, involucrin, loricrin, and filaggrin. (a) The figure shows a representative field of epidermis indicated by arrows immunostained brown with different antibodies against K1, involucrin, loricrin, or filaggrin and counterstained blue with hematoxylin. (b) Total cell lysates were isolated from the epidermis of mice. The protein levels of K1, involucrin, loricrin, and filaggrin in the lysates were determined by western analysis. K14 was used as a loading control