Fig. 3. zVAD differentially affects LPS- and poly I:C-induced signaling pathways: Inhibition of ERK and Akt, and enhancement of IRF3-STAT1 pathway, but no effects on IKK, JNK, and p38.

BMDMs were pretreated with zVAD (20 µM) for 30 min prior to stimulation with LPS (1 µg/ml) (A and C) or poly I:C (20 µg/ml) (B and D). After incubation for different periods as indicated, the total cell lysates were subjected to SDS-PAGE followed by immunoblotting for JNK, p38, IKK, ERK, Akt (A and B), IRF3, and STAT1 (C and D). The results were representative traces that were repeated in three independent experiments.