Skip to main content
. 2021 Dec 20;45(4):257–272. doi: 10.14348/molcells.2021.0193

Fig. 4. Effects of zVAD on LPS- and poly I:C-induced inflammatory gene expression and phagocytosis.

Fig. 4

(A-C) BMDMs were pretreated with zVAD (20 µM) prior to treatment with LPS (1 µg/ml) or poly I:C (20 µg/ml) for 6 h. Real time-PCR was conducted to measure gene expression. (D) Cells were treated with agents as mentioned above for 4 h and 8 h. E. coli bioparticles were treated at the last one hour. Phagocytosis was determined by flow cytometry. Data were the mean ± SEM from at least three independent experiments. *P < 0.05, indicating significant effects of TLR ligands. #P < 0.05, indicating significant effect of zVAD to inhibit the effects of TLR ligands.