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. 2021 Dec 20;45(4):257–272. doi: 10.14348/molcells.2021.0193

Fig. 7. ERK and Akt inhibition are not mediated by RIP1 and not involved in necroptosis.

Fig. 7

(A) BMDMs were pretreated with zVAD (20 µM) in the absence or presence of 3-MA (1 mM) or Nec-1 (10 µM) for 30 min and then treated with poly I:C (20 µg/ml). After incubation for different periods as indicated, the total cell lysates were subjected to SDS-PAGE followed by immunoblotting for ERK and Akt. (B) BMDMs were pretreated with U0126 (3 µM) or PD98059 (10 µM) for 30 min and then treated with LPS (1 µg/ml) and/or zVAD (20 µM). After 24 h, the cell viability was determined by MTT assay. veh, vehicle. Data were the mean ± SEM from at least three independent experiments. *P < 0.05, indicating synergistic cell death caused by LPS and zVAD.