Figure 4.

Monocyte transfer demonstrates retained inflammatory profile in male myeloid cells. Bone marrow monocytes from either female (F-F) or male (M-F) animals were next transferred into females that were then treated with 2 weeks of HFD. End-point assessments were made in (A) weight, (B) GWAT, % GWAT of total body weight, and (C) IWAT. Flow cytometry assessments of (D) ATM % of SVF, (E) donor ATM %, (F) donor-specific CD11c⁺ ATMs. (G) RT-PCR determined increased Mcp1 in animals given male monocytes. N = 11–12 per group. Data were analyzed by two-sample t-test. Data shown as average +/- SEM. *p < 0.05, **p < 0.01 and ***p < 0.005.