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. 2022 Mar 28;13:826320. doi: 10.3389/fendo.2022.826320

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Copyright © 2022 Varghese, Clemente, Lerner, Abrishami, Islam, Subbaiah and Singer

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sex differences in migration efficiency of monocytes with HFD. Monocyte trafficking (%) of BM monocytes from male and female (A) lean 22-week-old mice and (B) lean 22-week-old Tlr4^-/- mice. Monocytes stimulated with a combination of PA and MCP1 in (C) 2- to 4-week HFD mice, 6-week HFD mice, and 12- to 16-week HFD mice. N=4-6 mice per condition. Data were analyzed by 2-way ANOVA followed by Tukey’s multiple comparisons test. (D) Serum ELISA from ND, 2-, 6-, and 16-week HFD male and female of (D) MCP1 and CCL5. These data were analyzed by two-sample t-test. N = 12 for ND; 4 for HFD. Data shown as average +/- SEM. *p < 0.05, and ****p < 0.001. ns= non-significant.